Development of Borate Electrode for the Measurement of Glycosylated Proteins
Diabetes is a metabolic disease which requires a regular glisemic control in order to eliminate undesirable complications to improve and continue. Glisemic control can be executed from fasting blood glucose level measurement or measurement of glycated protein levels ,. There were many improvements made on measurement techniques of glycated proteins over the past 30 years. However these techniques reqiure professional staff, some preliminary procedures and they are expensive to execute. This project’s aim is to produce a highly borate selective electrode that can be used as a detector in the glycated protein measurement which will be used in diabetes diagnosis and monitoring. A micro scaled borate electrode was produced. This working electrode will be silverborate based composite solid electrode. Borate can make complex structures with glycated proteins therefore this compound can be used as a measurement technique. Developed borate selective electrodes were tested by a potentiometric reading in standing solutions varying from 1,0*10-1M and 1,0*10-5M. Developed borate selective electrode had a stable, linear and replicable potentiometric behaviour between 1,0*10-1M and 1,0*10-5M borate ion concentration. Developed electrode had selectivity to borate ions over sulfate, chloride, nitrate and some organic acid ions. Potentiometric readings of the electrode were consistent when pH was between 4 and 8 values. Developed electrode gave consistent readings through almost 3 months. Response time of the electrode was determined as 10 to 12 seconds. Response time being this low enhances this electrode’s reliability on fluid stream systems. Developed borate selective electrode is expected to be used in flow injections systems to help monitoring glycated protein levels in blood streams.