Determination of Recovery Rates of Legionella Bacteria by Different Methods

  • İpek ADA
  • Ayten KİMİRAN
Keywords: Legionella, semi-nested PCR, FISH, culture, recovery, water systems


Legionella are pathogenic bacteria that are present commonly in natural water and human-made water systems with 59 species and 70 serotypes and that causes Legionnaries’ disease and Pontiac fever. Legionella bacteria reproduce due to suitable conditions as they pass through the human-made water systems. In the present study, it was determined lowest detection limit of Legionella pneumophila by using conventional culture, fluorescent in situ hybridization (FISH) and semi-nested polymerase chain reaction (PCR) method in L. pneumophila ATCC 33152 bacteria and environmental L. pneumophila isolate at different concentrations (102 -1010 cell per liter). When the recovery results were evaluated; it was found that at least 103 cell per liter for culture method, 105 cell per liter for the FISH method and 106 cell per liter for semi-nested PCR method. For this reasons, it can be said that conventional culture method and molecular methods should be studied in support of each other.